TriKinetics果蝇行为监测系统

DAM2TriKinetics果蝇行为监测系统
TriKinetics果蝇行为监测系统

 

    Drosophila Activity Monitor (DAM) system能让广大的生物科学家的监测果蝇和类似大小的昆虫在运动和羽化方面的行为规律，此系统已被应用于突变异种、环境监测和对化学药品的敏感性，也可用来展示群居生活中相互间的作用。

 

果蝇被单独放置在玻璃瓶皿中，通过红外光束来探测和计算它们的运动行为，在一定的周期内，这些累计的行为数据将从监视器上传到计算机中存储和分析.

 

 

 

*运动监视器：用32个内径为 5mm,7mm或者10mm的管子，分别监测32个果蝇样本的行为规律。一个红外光束穿过把管子分成两半，当果蝇在管子中来回走动，它们将切割红外光，这些运动数据则被记录下来。

 

*羽化监视器：将成熟的果蝇虫卵粘贴在一个朔料圆盘底下，用一个内径100mm的玻璃漏斗来收集虫卵孵化出来的果蝇。一个红外监测环安装在漏斗下方的颈口处，用来监测计算每一个通过的果蝇，一个摇晃的机械装置可以定期的摇晃朔料圆盘，使得孵化出来的果蝇能够**从圆盘掉落到玻璃漏斗中。

 

*群聚监视器：用一个内径25mm的玻璃或者朔料小瓶子，装入多个果蝇，根据瓶子的长度放置几个红外监测环，记录果蝇运动总数。

 

*环境监测器：测量培养器皿内的光的强度，温度和相关的湿度

 

*电源：提供一个直流电源用于整个系统，通过一个电 话线网络一样的平行接口，这个电源可以提供给多达120个监视器同时使用

 

*记录软件：提供一个存储档案从一个或多个监视器同时记录行为数据。一个实验可连续记录数小时、数天甚至是几个星期。

 

 

 

 

 

The Drosophila Activity Monitoring System enables a biologist to accurately characterize the locomotor ａnd eclosion behavior rhythms in drosophila fruit flies ａnd similarly-sized insects.  The system has been used to screen for mutants, measure environmental ａnd chemical sensitivities, ａnd characterize social interaction, all based on the patterns of physical movement which the system observes ａnd quantifies over time.

 

The individual flies are placed into glass chambers, where their confined motion may be detected ａnd counted by infrared light beams in one or more activity monitoring units. At periodic intervals, these accumulated activity counts are uploaded from the monitors to a host Macintosh or Windows computer for storage ａnd analysis.

 

The Locomotor Monitor uses 32 tubes of 5, 7, or 10 mm diameter, ａnd measures the activity rhythms of 32 individual flies. An infrared beam bisects each tube, ａnd detects motion as the flies walk back ａnd forth from end to end.

 

The Eclosion Monitor uses a 100 mm dia. glass funnel to collect emerging flies as they fall from their pupae cases on the underside of a plastic disk. An infrared ring detector at the funnel neck counts each as it passes by, ａnd a mechanical tapper dislodges periodically those which remain adhered to the disk or funnel walls.

 

The Population Monitor uses a 25 mm dia. glass or plastic vial to contain a multiplicity of flies, ａnd measures their aggregate movement with ring detectors spaced along the vial length.

 

The Environment Monitor measures the light intensity, temperature, ａnd relative humidity within an incubator chamber, ａnd provides a continuous archival record of these conditions over the duration of an experiment. 

 

The Power Supply Unit supplies DC power to an entire system of up to 120 monitors using a parallel network of residence-type telephone cables. An optional Light Controller allows up to 6 incubator lights to be independently cycled on ａnd off under computer control.

 

The DAMSystem Collection Software provides archival storage of the collected activity data from a number of monitors simultaneously, ａnd allows for continuous operation over the hours, days, or weeks of an experimental run.

 

 

 

 

 

 

 

产品介绍

 

DAM5M果蝇活动监视器可同时测量32只果蝇的自发活动，可用于果蝇食物（food）、气味（odor）、方向（orientation）、灯光（light）等位置偏爱（position preference）实验，每个试管直径为5mm,当果蝇来回飞行时，只要中断试管1/4红外光束，就将有一个记录信号，每个试管有4个独立的红外光束，不但可以记录遗漏的数，而且红外到红外的移动杜绝了原地计数，还可记录在每个红外光束的停留时间（dwell time），内置安全的试管固定夹，易于移动和操作，全开放设计，试管灯光均匀渗透，无阴影。

 

 

 

特点

 

?8?432个试管用于32只果蝇

 

?8?4  直径5mm试管用于黑腹果蝇（drosophila Melanogaster）

 

?8?4  每个试管有4个独立的红外光束

 

?8?4  生成计数（Counts）、移动（Moves）、停留时间（Dwell）、休息（Rest）和每个果蝇的位置数据

 

?8?4  内置管夹用于快速加载和安全处理试管

 

?8?4  可折叠紧凑的存储和**试管

 

?8?4  操作一致的明暗灯光

 

?8?4  精密的管道布局

 

?8?4  电源，连接线和数据采集软件

 

?8?4  兼容DAM2,LAM25，DEnM和本公司其他监控单元

 

?8?3  产品规格

 

?8?4  试管直径：5mm

 

?8?4  试管长度：65mm或较大的

 

?8?4  尺寸： 33.0 x 8.3 x 2.6 cm

 

?8?4  质量：0.26 kg （不含试管）

 

?8?4  相邻红外间距：9.1mm

 

?8?4  连接： 4 wire, 6 position, RJ-11电话线接头

 

?8?4  9 v直流电源输入和数据传输

 

?8?4  数据采集软件：DAMSystem3，操作平台：Windows PC 或 Apple Macintosh

 

?8?4  数据类型：移动（Moves）、计数（Counts）、停留时间（Dwell）、位置（Position）,、休息（Rest）

 

?8?4  数据文件格式：42列 DAMSystem文本

 

?8?4  操作环境：正常实验室，不结露

 

 

 

 

 

The Drosophila Activity Monitor measures the activity rhythms of 32 individual flies, each in its own glass tube. In a typical experiment, a food mixture is placed into one end of the empty tube, ａnd sealed on the outside with a wax coating. The fly is then inserted into the tube, followed by a cotton plug or ventilated plastic cap on the other end. The tube is snapped into the plastic clips of the monitor case, ａnd if the tube is horizontal, an active fly will walk back ａnd forth from one end to the other. Each time that it does so, it breaks an infrared light beam which bisects the tube, perpendicular to its axis. This event is detected by the onboard electronics, ａnd added to the ｃount which is maintained for each channel. At periodic intervals, ranging from 1 second to 1 hour, the DAMSystem software in the host computer acquires from the monitor its set of 32 ｃount totals, ａnd saves them. This archival record of the activity of each fly over the duration of the experiment may be plotted or analyzed, ａnd used, for example, to measure the period ａnd phase of the internal circadian clock of each of the 32 flies. Such measurements have been instrumental to the scientific study of fly behavior, ａnd its genetic basis - the circadian clock being the cornerstone.  (See: THE TICK-TOCK OF THE BIOLOGICAL CLOCK. Michael W. Young in Scientific American, Vol. 282 No. 3, March 2000.)

 

 

 

Specifications

 

• Dimensions: 13.50 x 6.82 x 1.03”   (343 x 173 x 26 mm)

 

• Mass: 1.2 kg

 

• Glass Tubes: Corning Pyrex standard wall    tubing, according to model: DAM 5  =  5 mm diameter DAM10 = 10 mm diameter

 

• Maximum Tube Length: 3.00” (76 mm)

 

• Interconnect: 4 wire, 6 position, RJ-11 modular     telephone line jack to DAMSystem network for      DC power input ａnd data transmission

 

• Case material: PVC/ABS plastic 

 

 

 

Features

 

•32 independent activity channels per monitor.

 

•Up to 120 monitors per system provide over 3000 simultaneous monitoring channels.

 

•Models available for various tube diameters:    DAM 5   5 mm tube (for D. melanogaster)    DAM10 10 mm tube

 

•Built-in clips hold tubes in place during handling.

 

•Recessed tube mounts allow monitors to be safely stacked with tubes installed.

 

•Telephone-type wiring jack allows easy connection to DAMSystem wiring network.

 

•Consistent operation in ambient light levels from bright laboratory to total darkness.

 

•Field-proven design over years of continuous use.

 

•Optional accessories: Pyrex Glass Tubes     5 mm dia x 65 mm length    10 mm dia x 70 mm length

Trikinetics果蝇行为实时监测器技术参数：

 

尺寸：343×173×26mm

 

重量：1.2kg

 

玻璃管：康宁玻璃标准壁厚管（DAM5,直径5mm；DAM10,直径10mm）

 

玻璃管长度：76mm

 

外壳材料：PVC/ABS塑料

 

 

 

DAM2果蝇行为监控器（ Drosophila Activity Monitor）可测量32个果蝇的自发活动，每个都是一个独立的试管，可在试管里来自由的回飞行，果蝇穿过试管中点时就中断一个红外光束，这个中断信号将被控制板上的电路探测到，自动添加到试管的活动计数里，将被视为衡量果蝇飞行的一个测量。

 

在一个典型的实验里，琼脂/蔗糖食品混合物被放置在试管的一端，后面是果蝇飞行区域,棉花塞放在试管的开口端，32个试管是集中插在监控架上的。整个实验过程可能会持续数天或数周，在一定周期时段，计数电路将持续的监测32个试管的果蝇活动情况，实时上传数量总数到计算机进行存储和供后面的分析。每日记录数据为果蝇的自发活动强度和相对休息时间提供了一个很好的衡量标准。

 

DAM2是采用一个透明的聚碳酸酯塑料做成，允许光线沿着试管渗透到所有点，紧凑,重量较轻,当在孵化器里使用时，允许充足的空气环绕试管循环，监控架通孔设计允许任意长度的试管。每个单元上固定有一个开/关可见光传感器，可提供一个周围环境光线状态变化的简单记录，这个记录伴随活动计数数据获取和存储,在分析果蝇在昼夜节律和外部光刺激下的适应性是有用的。可选气体分布歧管，可以方便控制管内部空气交换,这主要用于缺氧研究（hypoxia studies）。

 

 

 

Trikinetics果蝇行为实时监测器特点：

 

1 每个32个独立的活动通道。

 

2 每个系统zui多可以安装120个监视器，同时检测3000个样本

 

3 两种型号适用于两种不同的管径的试管。

 

4 内置玻璃管夹持器

 

5 玻璃管易于安装

 

6 式接线插孔可方便地连接